Directed Enzyme Evolution: Screening and Selection Methods (Methods in Molecular Biology) by Frances H. Arnold and George Georgiou
English | ISBN: 158829286X | edition 2003 | PDF | 361 pages | 7,1 mb
English | ISBN: 158829286X | edition 2003 | PDF | 361 pages | 7,1 mb
Seasoned practitioners from many leading laboratories describe their best readily reproducible screening strategies for isolating useful clones. These techniques have been optimized for sensitivity, high throughput, and robustness, and are of proven utility for directed evolution purposes.
The assays presented use a variety of techniques, including genetic complementation, microtiter plates, solid-phase screens with colorimetric substrates, and flow cytometric screens.