Crispr Practical For Beginners: Hands-On Genome Editing

Design Your First CRISPR Project and Rewrite Code of Life Step-by-Step

What you'll learn

Design gene-specific primers with your own hands & feel the joy of creating your first tool for gene editing.

Run gel electrophoresis & feel the magic of seeing your invisible DNA become visible right in front of you.

Design your own CRISPR gRNAs & feel like a real gene engineer crafting tiny tools to edit life itself.

Perform Microinjection step-by-step to the first cell stage

Screen the CRISPR/Cas9 edit

Deasign your own CRISPR/Cas9 project and rewrite the code of life

Requirements

"I have designed this course with one simple dream — to make CRISPR Practical easy for everyone. No background required. No advanced lab needed. If you have a small lab and a curious heart, that’s more than enough to perform every experiment in this course.

Description

A step-by-step practical journey to design primers, perform PCR, run gels, design gRNAs,  do Microinjection, validate the CRISPR/Cas9 edits and carry out CRISPR gene editing — even if you’ve never worked in a lab before."This is This is not a typical biology theory  course filled with boring information and memorization.This is a hands-on lab experience where YOU perform CRISPR/Cas9 with your own hands"Welcome to CRISPR Practical for Beginners.What will you learn?→ Primer Designing — the first tool of every gene editor→ PCR — the heartbeat of molecular biology→ Gel Electrophoresis — watch your DNA become visible→ Principle of Gene Editing — the story of gene editing, CRISPR/Cas9 → gRNA Designing — create your CRISPR guide→ Microinjection — how to inject CRISPR/Cas9 to frist cell or zygote → CRISPR/Cas9 Gene Editing — perform real genome editing→ Mutation Screening — detect your edits like a scientistWhy take this course?Because skills last forever. Money comes back. Time comes back. But the confidence to say “I performed CRISPR myself” can change your career.Who is this for?Every student. Every beginner. Every dreamer who once thought: “I wish I could perform CRISPR too…” Yes, you can.RequirementsNo lab experience.No biology background.Just curiosity.Who am I?I am Faheem Kanwal — once a student with shaky hands and blurry gels. From a small lab, my journey led me to Shanghai Jiao Tong University and now to Tsinghua University China.  where I use CRISPR to develop future cancer therapeutics. I learned step by step, mistake by mistake — and now I’m here to teach you everything in the simplest, most practical way possible.My Promise to You"By the end of this course, I don’t want you to just say: “I know CRISPR.” I want you to proudly say: “I performed CRISPR with my own hands.”

Overview

Section 1: Welcome to the Practical World of CRISPR/Cas9

Lecture 1 Welcome

Lecture 2 The Story of Gene I

Lecture 3 The Story of Gene II

Lecture 4 The Story of DNA — The Language of Life

Lecture 5 What We Learned & What’s Ahead

Section 2: SnapGene, Heart of Molecular Biology

Lecture 6 What is SnapGene? DNA editor

Lecture 7 How you can download and install the SnapGene.

Lecture 8 A Story of " From Mom’s Loving Wraps to Life’s Clever Genes"

Lecture 9 The Art of Gene Retrieving: Your First Gene Map to Make Biology Click

Lecture 10 What We Learned & What’s Ahead

Section 3: The Art of Primer Designing

Lecture 11 Rules of Designing a Good Primer

Lecture 12 How You can design the primer of any Gene through SnapGene?

Lecture 13 How you can test and optimize the Your DNA Primers?

Section 4: DNA Extraction

Lecture 14 Fundamnetals of DNA extraction

Lecture 15 DNA Extraction from Zebrafish;Step By Step Hands On

Lecture 16 What is PCR and why we do it?

Lecture 17 The Concept of Gradient PCR; PCR Optimization

Section 5: From Invisible to Visible DNA : Step-by-Step Gel Electrophoresis

Lecture 18 What is Gel Electrophoresis and Why Do We Use It?

Lecture 19 The Concept of the DNA Ladder:Why do we use it?

Lecture 20 How to Prepare Your First Agarose Gel; Step by Step

Lecture 21 Lets Check the PCR band; Gel Documentation Step by step

Section 6: From Mutation to DNA Repair: The Genesis of Gene Editing

Lecture 22 Lecture 1: Typing Errors in the Book of Life: DNA Mutations Explained

Lecture 23 Who are genome guardians? : The Secrets of DNA Repair Systme

Lecture 24 Where It All Began Engineered Proteins and the Birth of Classical Gene Editing

Section 7: The Story of CRISPR/Cas9 Discovery

Lecture 25 The Story Of Gene Editing

Lecture 26 How did Jenniferobservation turn Imuunity concept to gen editing technology?

Lecture 27 CRISPR/Cas9 Components and How does it Work to Edit Genes

Section 8: Mastering gRNA Design for Successful CRISPR Experiments

Lecture 28 Designing gRNA Basics

Lecture 29 Desinging gRNA Manually Step By Step

Lecture 30 Designing gRNA Computationaly Step by Step

Lecture 31 Ordering gRNA online step by step guideline

Lecture 32 Synthesis of gRNA in lab stpe by step

Section 9: The Art of Microinjection

Lecture 33 Microinjection Introduction

Lecture 34 Viral and Non-Viral CRISPR Delivery Methods

Lecture 35 Microinjection; Prepration of Materials

Lecture 36 Microinjection; Injeting the gRNA/Cas9 into First cell stage Step by Step

For every dreamer who once thought — "I wish I could perform real gene editing too." This course is built for you.